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Definitions (431)
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cap site
In eukaryotes, the cap site is the position in the gene at which transcription starts, and really should be called the "transcription initiation site". The first nucleotide is transc [..]
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capsid
It is the external protein coat of a virus particle.
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riboprobe
A strand of RNA synthesized in-vitro (usually radiolabeled) and used as a probe for hybridization reactions. An RNA probe can be synthesized at very high specific activity, is single stranded (and the [..]
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unidirectional replication
It refers to the movement of a single replication fork from a given origin.
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absolute quantification
The absolute quantitation assay is used to quantitate unknown samples by interpolating their quantity from a standard curve. Adapter:
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adenine
One of the purine bases found in DNA and RNA (6-aminopurine), one member of the base pair A-T (adenine- thymine).
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alkaline phosphatase
An enzyme which catalyzes the hydrolysis of phosphomonoesters of the 5' nucleotides. Used to dephosphorylate (remove phosphate groups from) the 5' ends of DNA or RNA molecules, to facilitate [..]
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allele
An allele is one of the alternative (two or more) forms of a particular gene inherited separately from each parent; usually found at the same locus on homologous chromosomes. Equivalent genes in the t [..]
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allele-specific ligation
Technique permitting discrimination of two allele at locus by providing two short synthetic oligonucleotides that would bind adjacent to teach other on amplified DNA fragment, and would be ligated in [..]
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allelic discrimination assay
Assays designed to type for gene variants. Either differentially labeled (TaqMan®) probes (one for each variant) or a single probe and melting curve analysis can be used for this purpose. Alternative [..]
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