A series of procedures that are used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed from segments of two or more different DNA molecules. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate there, either on its own or after it has been integrated into a chromosome.

Methods involving the use of restriction enzymes to cleave DNA at specific sites, allowing sections of DNA molecules to be inserted into plasmid or other vectors and cloned in an appropriate host orga [..]

A technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vectors that carry the DNA into a host cell. In this host cell, the [..]

Procedures used to join together DNA segments in a cell-free system (e.g. in a test tube outside living cells or organisms). Under appropriate conditions, a recombinant DNA molecule can be introduced [..]

A breeding technique in which a copy of a DNA fragment containing one or more genes is transferred, or “recombined,” within another organism. Used widely in the cloning of genes, in genetic modification of organisms, and in molecular biology—including pharmaceutical production, generally.

A collection of experimental procedures that allow molecular biologists to splice a DNA

the technique by which genetic material from one organism is inserted into a foreign cell in order to mass-produce the protein encoded by the inserted genes.

Procedure used to join together DNA segments in a cell-free system (an environment outside a cell or organism). Under appropriate conditions, a recombinant DNA molecule can enter a cell and replicate there, either autonomously or after it has become integrated into a cellular chromosome.

The laboratory manipulation of DNA in which DNA, or fragments of DNA from different sources, are cut and recombined using enzymes. This recombinant DNA is then inserted into a living organism. rDNA te [..]

Techniques of gene cloning. Recombinant DNA refers to the molecule formed by joining a DNA of interest to vector DNA. See gene cloning.