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Definitions (269)
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query sequence
A sequence, either amino acid or nucleotide, chosen by the user to use in a database search. Two utilities, BLAST and FASTA, use a query sequence to perform searches. A query sequence can be typed or pasted into the the query window on the search form. Lengthy sequences can be copied after retrieval from the SGD Sequence form and pasted into the qu [..]
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point mutation
A single nucleotide change that substitutes one nucleotide for another. A point mutation in the coding sequence of a gene affects a single codon, and often allows expression of an intact but nonfunctional or partially functional protein.
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wildcard character
SGD uses an asterisk "*" as a wildcard symbol. In a search, the wildcard character shows where any text can be tolerated. For example, searching for the locus "cdc*" will produce all cdc genes. Searching for the Author "Johns*" will produce all authors whose last name begins with those letters.
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2-point data
This refers to data generated by tetrad analysis of a cross in which the segregation of two genetic markers is followed. These data yield the distance between the two markers (usually mutant alleles of genes) on the genetic map.
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5' utr intron
An intron located in the 5' prime untranslated region (SO:0000447).
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accession number
This refers to the unique GenBank identifier assigned to a sequence. This number can be used to search Genbank records for a specific sequence.
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acedb
AceDB was the database software previously used by SGD before the move to an ORACLE relational database. More information on AceDB is available here.
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affinity capture-ms
This term is used to identify and describe interaction data displayed at SGD. In this type of experiment, an interaction is inferred when a "bait" protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
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affinity capture-rna
This term is used to identify and describe interaction data displayed at SGD. In this type of experiment, an interaction is inferred when a "bait" protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by specific RNA binding.
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affinity capture-western
This term is used to identify and describe interaction data displayed at SGD. In this type of experiment, an interaction is inferred when a "bait" protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by Western blotting with a specific polyclonal [..]
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