An approach used to decode a genome by shredding ("shotgunning") it into smaller fragments of DNA which can then be individually sequenced. The sequences of these fragments are then ordered, based on overlaps in the genetic code, and finally reassembled into the complete sequence. The "whole genome shotgun" method is applied to [..]

Shotgun sequencing is a laboratory technique for determining the DNA sequence of an organism's genome. The method involves breaking the genome into a collection of small DNA fragments that are sequenced individually. A computer program looks for overlaps in the DNA sequences and uses them to place the individual fragments in their correct orde [..]

A way of determining the sequence of a large DNA fragment which requires little brainpower but lots of late nights. The large fragment is shotgun cloned (see above), and then each of the resulting smaller clones ("subclones") is sequenced. By finding out where the subclones overlap, the sequence of the larger piece becomes apparent. Note [..]

A method of sequencing genomes and environmental samples in which random fragments of DNA are sequenced and then computational methods are used to “reassemble” genomes from the sample.

The sequencing of a large DNA segment through the sequencing of randomly-derived subsegments whose order and orientation within the large segment is unknown until the assembly of overlapping sequences. The method works if all positions in the large segment are covered by multiple overlapping subsegments. See also the entry at NHGRI's Talking G [..]

(genetics) A DNA sequencing technique in which a large number of small fragments of a long DNA strand are generated at random, sequenced, and reassembled to form a sequence of the original strand. [..]