A technique in molecular biology that is used to separate and identify particular

A laboratory test for specific antibodies to confirm repeatedly positive results on the HIV ELISA or EIA tests. In the United States, Western blot is the validation test used most often for confirmati [..]

Western blotting is a laboratory technique used to detect a specific protein in a blood or tissue sample. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred out of the gel to the surface of a membrane. The membrane is exposed to an antibody specific to the target protein. Bin [..]

A Western blot is a laboratory method used to detect specific proteins from a mixture of proteins © Nature Education

A technique for detecting specific proteins using an antibody. Proteins are separated by electrophoresis in an acrylamide gel (PAGE), and transferred onto a membrane. The membrane is then probed with [..]

a technique of identifying a specific protein molecule within a mixture.

A technique for analyzing mixtures of proteins to show the presence, size and abundance of one particular type of protein. Similar to Southern or Northern blotting (see "Blotting"), except that (1) a protein mixture is electrophoresed in an acrylamide gel, and (2) the "probe" is an antibody which recognizes the protein of intere [..]

A method of detecting a particular protein molecule in a tissue or organ, by separating proteins from that source with gel electrophoresis, blotting the separated proteins onto nitrocellulose, and then using an antibody that binds, and highlights, the protein of interest. Compare Northern blot and Southern blot.

Similar in principle to a Southern blot, but where the species adsorbed to the nitrocellulose filter is a protein, and the detection makes use of specific antibodies.

A confirmation test for the presence of specific antibodies that is more accurate than the ELISA test for detecting true negatives.   XClick here to return to list   YClick here to return to list   Z [..]

A type of antibody test used to confirm a positive result on an HIV screening test. (The initial screening test is usually a different type of antibody test or, less often, a viral load test). The immune system responds to HIV infection by producing HIV antibodies. A Western blot for confirmatory HIV testing is done using a blood sample. See Relate [..]

A procedure in which a mixture of proteins is separated on a polyacrylamide gel and then transferred to a nylon membrane. The membrane may then be treated with reagents such as specific antibodies [..]

A form of immunoblot

A technique for the separation, immobilization, and detection of proteins, usually by a labeled antibody.

An assay that detects specific proteins within a protein mixture. Samples are subjected to electrophoresis on a slab gel. A replica of the gel is then made on a membrane by electrophoretic transfer. Specific proteins are then detected on the membrane using antibody staining.

A blood test used to detect antibodies to HIV; used to confirm or supplement positive EIA/ELISA results.

a laboratory testing method. A form of Western blot is reactive to HIV, and is used to confirm presence of HIV antibody as detected by ELISA. However, there has been considerable controversy in the medical literature about the correct interpretation of Western blot results.